A procedure used in IVF fertility programs, particularly in cases of male infertility where no sperm is found in the ejaculate (azoospermia), either due to obstruction (blocked sperm ducts) or non-obstructive causes (sperm production issues).1. PESA (Percutaneous Epididymal Sperm Aspiration) is a technique for retrieving sperm from the epididymis (a duct above the testis where sperm matures) using a fine needle through the skin without major surgery. It is typically performed in patients with obstructive azoospermia, such as men with blocked sperm ducts.2. TESA (Testicular Sperm Aspiration) involves retrieving sperm directly from the testicular tissue using a needle. This method is used if PESA fails or if no sperm is found in the epididymis.3. MESA (Microsurgical Epididymal Sperm Aspiration) is the retrieval of sperm from the epididymis using a surgical microscope (microsurgery). It allows for the collection of a larger quantity and higher quality sperm, usually indicated for obstructive azoospermia, especially when a large number of sperm is needed for freezing or further IVF procedures.